Production of medakafish chimeras from a stable embryonic stem cell line
نویسندگان
چکیده
منابع مشابه
Production of medakafish chimeras from a stable embryonic stem cell line.
Embryonic stem (ES) cell lines provide a unique tool for introducing targeted or random genetic alterations through gene replacement, insertional mutagenesis, and gene addition because they offer the possibility for in vitro selection for the desired, but extremely rare, recombinant genotypes. So far only mouse blastocyst embryos are known to have the competence to give rise to such ES cell lin...
متن کاملProduction of zebrafish germ-line chimeras from embryo cell cultures.
Although the zebrafish possesses many characteristics that make it a valuable model for genetic studies of vertebrate development, one deficiency of this model system is the absence of methods for cell-mediated gene transfer and targeted gene inactivation. In mice, embryonic stem cell cultures are routinely used for gene transfer and provide the advantage of in vitro selection for rare events s...
متن کاملPluripotency and differentiation of embryonic stem cell lines from the medakafish (Oryzias latipes)
Small aquarium fish, like the medaka and zebrafish, offer an excellent opportunity to combine embryological, genetic and molecular analyses of vertebrate development. Pluripotent embryonic stem (ES) cells have enormous potential to study the totipotency and differentiation of cells and provide s bridge linking in vitro manipulations of the genome. In this report we describe the establishment, p...
متن کاملproduction of buffalo embryonic stem cell from hmc embryos
embryonic stem cells (escs) are derived from the inner cell mass (icm) of blastocyst and differentiate into all three embryonic germ layers: ectoderm, endoderm, and mesoderm. in this study, escs are derived from hand made cloning (hmg) blastocysts and their efficiencies compared to escs derived from in vitro fertilization (ivf) embryos. feeder layer was used for escs culture, and culture medium...
متن کاملSimple and efficient production of embryonic stem cell-embryo chimeras by coculture.
A method for the production of embryonic stem (ES) cell-embryo chimeras was developed that involves the simple coculture of eight-cell embryos on a lawn of ES cells. After coculture, the embryos with ES cells attached are transferred to normal embryo culture medium and allowed to develop to the blastocyst stage before reimplantation into foster mothers. Although the ES cells initially attach to...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1998
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.95.7.3679